96-Well ROCK Activity Assay Kit
| Specifications | |
|---|---|
| Product Category: | Rho Kinase (ROCK) Assays |
Product Description
Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20).
The 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.
Product Citations
- Burban, A. et al (2017) Penicillinase-resistant antibiotics induce non-immune-mediated cholestasis through HSP27 activation associated with PKC/P38 and PI3K/AKT signaling pathways. Sci Rep. (1):1815.
- Kaplan, H.M. et al. (2017) Effects of chronic Δ9-tetrahydrocannabinol treatment on Rho/Rho-kinase signalization pathway in mouse brain. Saudi Pharmaceut. doi:10.1016/j.jsps.2017.05.002.
- Silveira, A.A.A., et al (2017) TNF induces neutrophil adhesion via formin-dependent cytoskeletal reorganization and activation of β-integrin function. J Leukoc Biol. doi: 10.1189/jlb.3A0916-388RR.
- El Azreq, M. A. et al. (2016) Discoidin domain receptor 1 promotes Th17 cell migration by activating the RhoA/ROCK/MAPK/ERK signaling pathway. Oncotarget. doi:10.18632/oncotarget.10455.
- Munoz, A. et al. (2016) Aging-related increase in Rho kinase activity in the nigral region is counteracted by physical exercise. J Gerontol. 71:1257-1257.
- Catalog Number
STA-416-CB - Supplier
Cell Biolabs - Size
- Shipping
Blue Ice
Price
957,00 €
