ROCK Activity Immunoblot Kit
| Specifications | |
|---|---|
| Product Category: | Rho Kinase (ROCK) Assays |
Product Description
Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20).
The ROCK Activity Immunoblot Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates.
Product Citations
- Seo, J. H. et al. (2016) Dynamic polyrotaxane-coated surface for effective differentiation of mouse induced pluripotent stem cells into cardiomyocytes. RSC Adv. doi:10.1039/C6RA03967G.
- Tocci, P. et al. (2016) Endothelin-1/endothelin A receptor axis activates RhoA GTPase in epithelial ovarian cancer. Life Sci doi:10.1016/j.lfs.2016.01.008.
- Watts, B. A. et al. (2016) High mobility group box 1 inhibits HCO3-absorption in medullary thick ascending limb through RAGE-Rho-ROCK-mediated inhibition of basolateral Na+/H+ exchange. Am J Physiol Renal Physiol. doi:10.1152/ajprenal.00185.2016.
- Gross, P. et al. (2015) Para-cresyl sulfate acutely impairs vascular reactivity and induces vascular remodeling. J Cell Physiol. doi: 10.1002/jcp.25018.
- Su, C. C. et al. (2015) Phenotypes of trypsin-and collagenase-prepared bovine corneal endothelial cells in the presence of a selective Rho kinase inhibitor, Y-27632. Mol Vis. 21:633-643.
- Catalog Number
STA-415-CB - Supplier
Cell Biolabs - Size
- Shipping
Blue Ice
Price
676,00 €
