Rac1 Activation Assay

Product Description

Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.

Rac1, a 21 kDa protein, belongs to the family of Rho GTPases regulating a variety of biological response pathways that include cell motility, cell division, gene transcription, and cell transformation. Like other small GTPases, Rac1 regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Rac1 binds specifically to the p21-binding domain (PBD) of p21-activated protein kinase (PAK) to control downstream signaling cascades.

Cell Biolabs’ Rac1 Activation Assay Kit utilizes PAK PBD Agarose beads to selectively isolate and pull-down the active form of Rac from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rac is detected by western blot analysis using an anti-Rac1 specific monclonal antibody.

Cell Biolabs’ Rac1 Activation Assay Kit provides a simple and fast tool to monitor the activation of Rac1. The kit includes easily identifiable PAK1 PBD Agarose beads, pink in color, and a Rac1 Immunoblot Positive Control for quick Rac1 identification. Each kit provides sufficient quantities to perform 20 assays.