Pan-Ras Activation Assay

Product Description

Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Ras, a 21 kDa protein, regulating a variety of biological response pathways that include cell growth, cell transformation and tumor invasion. Like other small GTPases, Ras regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Ras binds specifically to the Ras-binding domain (RBD) of Raf1 to control downstream signaling cascades. The most notable members of the Ras subfamily are H-Ras, N-Ras and K-Ras, mainly for being implicated in many types of cancer.

Cell Biolabs’ Pan-Ras Activation Assay Kit utilizes Raf1 RBD Agarose beads to selectively isolate and pull-down the active forms of Ras (H-Ras, K-Ras, and N-Ras) from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Ras is detected by western blot analysis using an Anti-pan-Ras Antibody.

Cell Biolabs’ Pan-Ras Activation Assay Kit provides a simple and fast tool to monitor the activation of Ras. The kit includes easily identifiable Raf1 RBD Agarose beads (Figure 1), pink in color, and a GTPase Immunoblot Positive Control for quick Ras identification.

Each kit provides sufficient quantities to perform 20 assays.