Stellaris® FISH Probes, Mouse Neat1 Middle Segment with Quasar® 570 Dye

Product Description

Stellaris® FISH Probes,Mouse Neat1_m consists of a set of Quasar® 570-labeled oligos mixed at equal ratios and pooled into a final delivered amount of 1 nmol, which yields approximately 80 hybridizations under standard conditions.

Designed to detect Neat1_m transcripts in Mouse specimens using fluorescence in situ hybridization (FISH).

Design Criteria

Product was designed against Mouse nuclear paraspeckle assembly transcript 1 (non-protein coding), Neat1_m, a.k.a. VINC; 2310043N10Rik (NCBI gene ID: 66961), and the middle of the long variant of GQ859163 nts 4001-12000.

The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.

Fluorescence Parameters

• Absorption Maximum (Lambda Max): 590 nm
• Fluorescence Maximum: 610 nm

Product Usage

Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles.

For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.

For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.

Protocols

Detailed protocols by sample type can be found here.