Stellaris® FISH Probes, Custom Assay with Biotin Label

Custom Stellaris® RNA FISH Probe Set labeled with Biotin to detect your RNA of interest. 

Product Description

Stellaris RNA FISH is a RNA visualization method that allows simultaneous detection, localization, and quantification of individual RNA molecules at the cellular level in fixed samples using fluorescence microscopy.

A set of Stellaris RNA FISH Probes is comprised of a pool of up to 48 unique probes, each labelled with a fluorophore, that collectively bind along an RNA target transcript to produce a diffraction limited spot.

Before considering designing a Stellaris RNA FISH Customized Probe Set, please also check out our Stellaris RNA FISH DesignReady Probe Sets for a large selection of professionally designed targets.

We also recommend selecting a Stellaris RNA FISH Positive Control (ShipReady) Probe Set RNA  to complement your experiment. 

Stellaris Buffers are the perfect accompaniment to your experiment, allowing for even more robust RNA detection and analysis through reduced background signal. 

Biotin Label

The biotin modification incorporates a biotin functionality at the 3' terminus of an oligonucleotide. Biotin binds to avidin or streptavidin conjugates in a variety of fluorescent, chemiluminescent and colorimetric assays.

Special Notes and Considerations: Biotin labeling enables you to gain more flexibility on labeling options for your Stellaris RNA FISH probes.

We will deliver the Stellaris RNA FISH probes to you with only the biotin modification, and you would then apply any desired label yourself. This process can be challenging, and generally results in about a 50% loss of material and should be avoided if one of our pre-labeled probe sets will fulfill your needs. Further, biotinylated probe sets, which require secondary detection also may increase both false positive and negative detection rates of RNAs, partly due to endogenous biotin in cells and tissue.

Please note that Stellaris RNA FISH probe sets tagged with biotin should not be used for ChIRP or any other RNA pull-down method relying on biotinylated oligos. 

C3 (Biotin); 3' Modification product information

Properties:

• Name: Biotin
• Formula: C₁₃H₂₂N₃O₄S

Design considerations

Please read the tech article titled, “Designing Stellaris RNA FISH Probes” before designing your custom assay.

You can also use Chroma’s Spectra Viewer to check your microscope’s dye filter set compatibility. Use the Stellaris Dyes and Modifications page to select the right dye for your microscope and multiplexing recommendations.

Note I: Due to the variable autofluorescence in the green channel inherent to some cells and tissues, it is not recommended to use FAM for some Stellaris RNA FISH assays. We recommend testing your sample for green channel autofluorescence before ordering FAM labeled sets or ordering an alternative dye that is compatible with your experiment.

Note II: The success rate of Custom Probe Sets depend on many variables, such as imaging platform, operator use of optics, target sequence, transcript number, subcellular localization of transcript, number of probes in the set, space between probes, masking level, sample identity whether cultured cells or fresh or frozen tissue, preparation and handling of sample, and customer's design of probe sets with or without the Stellaris Probe Designer. The customer is responsible for designing a probe set that is compatible with all of the variables mentioned above unique to their platform technologies and model system, and therefore we cannot guarantee the success of Customer Probe Sets, or troubleshoot the designs.

Please contact us by email, phone or using this form for further advise and details.