6-FAM Calibration Standard (T10)

Product Description

Calibration is required by many real-time thermal cyclers in order to generate a spectral profile for the fluorescent signal. Dye information is stored within the instrument’s software settings, and is used to control bleed-through into adjacent channels. The analysis software anticipates how much fluorescence to expect from each dye during amplification, and will subtract out signal from inappropriate filter-sets. This crosstalk is particularly consequential to a multiplexed assay, since multiple targets are amplified simultaneously but detected independently. Different fluorescent reporters are used to signal each target and so calibration with dye standards allows the instrument to resolve their overlapping spectra.

On certain instruments, all dyes that will be multiplexed together must be calibrated together, and so a FAM standard is available as well.

Calibration Instructions

The number and specification of instruments are always changing. Therefore, if you don´t find information on your instrument in the Calibration Instruction Sheet please feel free to contact us.