BlazeTaq SYBR Green qPCR Mix 2.0 (with ROX)

For universal quantitative real-time PCR

Product Description

BlazeTaq™ SYBR® Green qPCR Mix 2.0 is designed for highly sensitive and accurate quantification of gene expression and real-time PCR reactions. It contains a hot-start antibody-modified Taq DNA polymerase and optimized buffer system that avoids non-specific amplification of target DNA at lower temperatures, and enhances reaction mix performance. The reaction kit offers ready-to-use 5X Master Mix- just add the DNA template, primers and deionized water to start your fast and specific quantitative analysis.

• Faster heat activation, 30s vs 15 min
• Less heat-damage to DNA samples
• More stable performance
• Detects as little as 5 copies of DNA template
• High specificity with minimal level of primer-dimer & non-specific product formation
• High amplification efficiency over wide GC-content range

The BlazeTaq™ SYBR® Green qPCR Mix 2.0 can be used in combination with the SureScript™ First-Strand cDNA Synthesis Kit as part of the two-step real-time RT PCR system.

Features

• 2X Master Mix for convenient use
• Hot-start polymerase and optimized buffer system to reduce non-specific reactions
• Available with or without ROX for use with all common qPCR instruments

Figure 1: Wide Dynamic Range with High Sensitivity DNA templates ranging from 5×10^7 (blue) to 5 copies (orange) were amplified. Linear regression of fluorescent signal on cycle numbers shows R2 of 99.8% and amplification efficiency of 98.8%.

Figure 2: Reaction Efficiency

Figure 3: Amplification of the B2M gene using serial diluted cDNA from HeLa cell line Performance comparison of BlazeTaq™ (red) with BioRad SsoAdvanced™ Universal SYBR® Green Supermix (blue) shows stronger signals with similar amplification performance.

Figure 4: Performance comparison of BlazeTaq™ (red) with PowerUp™ SYBR Green Master Mix from Applied Biosystems (green), and GoTaq® qPCR Master Mix from Promega (blue) showed greater sensitivity, amplification efficiency and specificity

Figure 5: Ct value comparison Amplification of 41 genes from 10 ng of HeLa cDNA using BlazeTaq™ (blue), PowerUp™ SYBR Green Master Mix from Applied Biosystems (orange), and GoTaq® qPCR Master Mix from Promega (grey).

Figure 6: Consistent detection of low copy number DNA templates Ct values shown for 50 and 5 copies of DNA templates, and no template control (NTC). For each template, the qPCR reactions were repeated 24 times.

Figure 7: Amplification efficiencies range from 90% to 110% over a wide range of GC content. DNA templates with different GC-content were serial-diluted and amplified.