pCDH-EF1α-CymR-T2A-Puro SparQ CymR Expression Vector

Product Description

With SBI’s SparQ™ Cumate Switch System, you can get inducible gene expression in mammalian cells through the binding of cumate, a non-toxic small molecule, to CymR. CymR can be delivered to cells on its own lentivector.

Deliver the CymR repressor for tight, titratable control of SBI’s SparQ Lentivectors – drive CymR with the EF1α promoter and select with a puromycin marker. The SparQ System is characterized by:

• Robust—increase expression up to 32-fold
• Adjustable—tune expression levels by titrating the amount of cumate
• Reversible—turn expression on, then off, then on again
• Versatile—choose from all-in-one formats that co-express CymR and your gene-of-interest, or two-vector systems where CymR is expressed from a different plasmid
• Powerful—suitable for in vivo applications

How it works

The regulatory mechanisms of the bacterial operons cmt and cym have been engineered to regulate gene expression in mammalian cells. In the repressor configuration, regulation is mediated by strong binding of the CymR repressor to the Cumate operator site (CuO), which is downstream of the CMV5 promoter. Addition of cumate (depicted as yellow circle in the graphic scheme below), a non-toxic small molecule inducer, relieves the repression and drives gene activation; in this case, fluorescent proteins (RFP and GFP). Subsequent removal of cumate from the growth media reverts the activation process, rendering gene switches to off mode.

Figure 1. Cumate Switch System