Cumate Solution (1000x) for SparQ Systems, 500ul

Product Description

Control the cumate-inducible promoter used in SBI’s SparQ Lentivectors with this 1,000x inducer solution.

With SBI’s SparQ™ Cumate Switch System, you can get inducible gene expression in mammalian cells through the addition of cumate, a non-toxic small molecule. Expression levels are tightly controlled and increase with increasing cumate concentration until maximum induction is reached—see as much as a 32-fold increase in gene expression. Even better, induction is reversible, so you can turn expression levels on and off. Delivering negligible background expression in the absence of cumate, the SparQ System is an excellent choice for achieving controlled levels of gene expression.

A separate manual is available for use with the SparQ2^TM Cumate Switch System.

How it works

The SparQ Cumate Switch System delivers robust, titratable gene expression with low background through three components:

• Cumate, a non-toxic, small-molecule inducer
• CymR, a repressor that binds to cumate operator sequences in the absence of cumate
• SparQ Lentivector that contains an MCS to clone-in your gene-of-interest, the cumate inducible promoter with cumate operator sequences (CuO) upstream of the MCS, and one or more markers

CymR has a high binding affinity for cumate and, as more cumate is added, fewer CymR molecules bind to the CuO sequences in the promoter resulting in increased expression. Exhibiting much lower background expression than similar systems, SBI’s cumate-inducible vectors can provide up to 32-fold induction of gene expression.