PiranhaTM Electroporation-ready GFP-tagged mRNA (10µg)

Product Description

Quickly and directly study the role a protein plays in vivo with SBI’s Piranha Targeted Protein Degradation System, Electroporation-ready mRNA. After electroporating both the Piranha mRNA and an antibody specific for your protein-of-interest into target cells, you can start seeing degradation of the protein-of-interest in as little as 1 hour*. Piranha Electroporation-ready mRNA is available in untagged, RFP-tagged, or GFP-tagged versions (also available as Pre-packaged Lentivirus or HEK293T Stable Cell Line).

SBI’s Piranha Targeted Protein Degradation System is based on the TRIM21 pathway found in most mammalian cells. Like TRIM21, the Piranha protein binds to conserved regions of an antibody, targeting the antibody—and the bound antigen—for degradation by the proteasome.

*Based on published data by Clift et al., 2014

To use Piranha Electroporation-ready mRNA for targeted protein degradation, simply electroporate the Piranha mRNA and a validated antibody specific for the protein-of-interest into target cells, wait an appropriate amount of time, and then observe the phenotypic outcome.

Because the rate of protein degradation is based upon many factors, we recommend performing initial experiments to empirically determine the degradation rate of your target protein and the optimal time for phenotypic observation.

Reference

1. Clift D, et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell. 2017 Dec 14;171(7):1692-1706.e18. doi: 10.1016/j.cell.2017.10.033.