PromoterTest™ Assay Custom Kit (25 ug/construct)

Product Description

The PromoterTest™ Assay Custom Kit enables you to choose from a range of promoter constructs to identify the promoter that is optimally suited to express a cDNA of interest in your mammalian cell system. With Cellecta’s PromoterTest Assay Custom Kit you can:

• Pick and choose from a set of promoter constructs, paying only for what you need
• Test them in your cell system to assess relative promoter strength
• Identify an optimal promoter for your mammalian cell line by FACS for any application requiring robust expression of a gene of interest

You have a choice of 10 lentiviral constructs to include in your PromoterTest Custom Assay Kit. Each lentiviral vector carries the same green fluorescent protein (GFP) under the control of a promoter. 

Cellecta's PromoterTest reporter constructs include a range of commonly used promoters. Transduce the GFP-expressing lentiviral constructs into your cell line and compare the GFP intensities from each different construct. For more convenience we can also deliver pre-packaged ready-to-transduce lentiviral particles. The intensity of the resulting fluorescence, as measured by FACS, correlates with the expression level of GFP, with higher GFP intensity indicating stronger promoter activity.

Please contact us to order your choice of constructs. Price per construct decreases as you order more constructs.

With this customizable kit, you can now measure the relative expression level of multiple promoters by assessing promoter strength (Fig. 1) as well as sustained long-term expression (Fig. 2) to find an optimal promoter for virtually any mammalian cell line.

Supporting Data

Fig. 1 Relative expression levels of GFP by CMV, mCMV, SSFV, E/CMV, EFS and RSV promoters in various cell lines. (Data normalized by average expression in a cell line.)

Fig. 2 FACS readout shows that EF1L sustained the highest expression in this Induced pluripotent Stem Cell (iPSC) line.