Empty PinPoint Donor Vector (pPP-MCS-WPRE-pA-attB-Puro-pA)

• Create isogenic cell lines
• Achieve high-efficiency integration with no insert size-limit
• Turn cell line construction into a high-throughput process

With the PinPoint Targeted Integration System you can get controlled, single-copy integration of your PinPoint Donor Vector at a pre-placed PinPoint attP site. The Empty PinPoint Donor Vector (pPP-MCS-WPRE-pA-attB-Puro-pA) enables integration of any expression cassette or other sequence for complete customization ability. The vector also includes a promoterless puromycin marker to enable selection for the desired integrants—proper integration into a PinPoint attP site inserted using SBI’s PinPoint Placement Vector results in a PGK promoter positioned to drive the puromycin marker, enabling selection for the desired integrants. The vector also includes a LoxP site to mediate removal of most of the unnecessary vector sequences and both kanamycin and hygromycin markers for cloning and propagation.

With the PinPoint Targeted Integration System, you can ensure that only a single copy of your transgene is inserted into the genome.

Learn more about Targeted integration with the PinPoint System