EF1alpha PinPoint Donor Vector (pPP-EF1a-MCS-pA-attB-Puro-pA)

Easy and Efficient Isogenic Cell Line Creation

Product Description

Deliver your gene-of-interest with this PinPoint Donor Vector – get single-copy integration at your pre-placed attP site and express your cDNA with EF1α

• Create isogenic cell lines
• Achieve high-efficiency integration with no insert size-limit
• Turn cell line construction into a high-throughput process

With the PinPoint Targeted Integration System you can get controlled, single-copy integration of your PinPoint Donor Vector at a pre-placed PinPoint attP site. The EF1α PinPoint Donor Vector (pPP-EF1α-MCS-pA-attB-Puro-pA) uses the moderate EF1α promoter to drive your gene-of-interest, and includes a promoterless puromycin marker—proper integration into a PinPoint attP site inserted using SBI’s PinPoint Placement Vector results in a PGK promoter positioned to drive the puromycin marker, enabling selection for the desired integrants. The vector also includes a LoxP site to mediate removal of most of the unnecessary vector sequences and both kanamycin and hygromycin markers for cloning and propagation.

With the PinPoint Targeted Integration System, you can ensure that only a single copy of your transgene is inserted into the genome.

Learn more about Targeted integration with the PinPoint System