Monkeypox Virus 1-Step High Throughput PCR Kit

Product Description

• Monkeypox virus specific / no cross-reacitvity with other Orthopox viruses.
• Hot-start Taq DNA Polymerase with high efficiency and fidelity.
• Double quencher probe ensures enhanced specificity.
• UDG and dUTP system minimizes carry-over contamination.
• ROX reference dye improves accuracy and reproducibility.

The RayBiotech Monkeypox Virus 1-Step High Throughput PCR Kit is a ready to use PCR assay for the detection of MPXV DNA. It uses a fluorescent probe with specific primer sets to detect the J2R gene specific for the genome of MPXV. Primer and probe for an internal control, RNase P are also integrated in the assay to validate the assay quality. The kit also contains a proprietary buffer which enables the user to use crude skin swabs directly in their PCR reactions. By simply mixing the 1-Step Nucleic Acid Stabilization Buffer with the sample, the viral DNA is immediately released and stabilized, and interfering components of the human tissue are inactivated. This eliminates the need for laborious, costly DNA extraction and purification steps, drastically improving upon the traditional workflow of PCR, while removing potential sample loss and contamination.

Assay Format

Sensitivity: 4 copies / µl
Specifity: No cross reactivity was identified when the kit was evaluated against other common Orthopox family members: Cowpox virus, Camelpox virus, and Vaccinia virus.
Number of Targets Detected: 1
Species Detected: Virus
Compatible Sample Types: DNA
Method of Detection: PCR
Quantitative/Semi-Quantitative: Qualitative

Protein Information

Accession Number: O57108
Gene Symbols: J2R
Protein Name & Synonyms: J2R (J2L)

This test is for Research Use Only (RUO).

Not for diagnostic or therapeutic use.