Primary Cells Detach Kit

Product Description

The Primary Cells Detach Kit contains the following reagents for primary cells detach and subculture:

• 500 ml DPBS (Dulbecco’s Phosphate-Buffered Saline)
• 100 ml T/E Solution (Trypsin / EDTA solution)
• 100 ml TNS (Trypsin Neutralization Solution)

Component Description

Dulbecco’s Phosphate-Buffered Saline

is a commonly used buffer in biological research. DPBS helps maintain a constant pH. The solution does not conatin Ca2+ or Mg2+ and is sterile-filtered. Because it is not toxic to cells and is isotonic, it may be used to rinse cells without causing any damage to them.

Trypsin / EDTA solution (T/E)

is a sterile, phosphate and HEPES-buffered saline solution. It contains 0,25% trypsin, 0,5 mM EDTA, 1 mM sodium pyruvate and 10 mM HEPES. This product has a pH of 7.4 at room temperature. Trypsyn/EDTA Solution is used to detach adherent cells from a culture surface.

Trypsin Neutralization Solution (TNS)

is a sterile, phosphate and HEPES-buffered saline solution. It contains 10% fetal bovine serum as a trypsin inhibitor and cell protect agents. The product is calcium- and magnesium-free and has a pH of 7.4 at room temperature. Trypsin Neutralization Solution is used to neutralize the effects of Trypsin/EDTA solution after the release of cells from a culture surface.