EpiQuik In Situ DNA Damage Assay Kit

Product Description

The EpiQuik™ In Situ DNA Damage Assay Kit is a convenient set of tools that allows the experimenter to detect DNA damage or apoptosis by measuring phosphorylation of H2AXSer139 in situ. The kit is ready-to-use and provides all the essential components needed for specifically measuring DNA damage in situ through phospho H2AXSer139 detection using cultured adherent cells. The kit has the following advantages:

• Quick and efficient procedure, which can be finished within 3 hours
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography
• Measurement of in situ histone H2AX phosphorylation without the need to prepare cell lysates
• Microplate format makes the assay suitable for high throughput analysis of agents that increases or inhibits DNA damage
• Simple, reliable, and consistent assay conditions

Principle & Procedure

EpiQuik™ In Situ DNA Damage Assay Kit is a whole cell-based detection of DNA damage and/or apoptosis. In this assay, adherent cells are cultured in conventional 96 -well microplates. After your experimental treatment, cells are fixed and permeabilized. The phosphorylation of H2AX at serine139, the sensitive marker of DNA damage, is then detected by an anti-phospho H2AXSer139 antibody. The ratio or amount of phospho H2AXSer139 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

Performance Data

Fig.1. MCF-7 cells in microplate wells were exposed to etoposide for 4 h and DNA damage was detected by the measuring level of phosphor-H2AX.