ChromaFlash One-Step Magnetic ChIP Kit

Identify the DNA Binding Partners of Transcription Factors or Histones in Chromatin

Product Description

The ChromaFlash™ One-Step Magnetic ChIP Kit is a complete set of optimized buffers and reagents, using a magnetic beads-based method, to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell.

Principle & Procedure

The ChromaFlash™ One-Step Magnetic ChIP Kit contains all necessary reagents required for carrying out a successful chromatin immunoprecipitation directly from chromatin extracts isolated from mammalian cells or tissues.

This kit includes a positive control antibody (RNA polymerase II), a negative control non-immune IgG, and GAPDH primers that can be used as a positive control to demonstrate the efficacy of the kit reagents and protocol. RNA polymerase II is considered to be enriched in the GAPDH gene promoter that is expected to be undergoing transcription in most growing mammalian cells and can be immunoprecipitated by RNA polymerase II but not by non-immune IgG. Immunoprecipitated DNA is then cleaned, released, and eluted. Eluted DNA can be used for various downstream applications such as ChIP-PCR, ChIP-on-chip, and ChIP-seq.