TGEX-HC-hG1[NA]-Zeo - Plasmid for Human IgG1 [NA] Mutant

Product Description

Heavy Chain Cloning Vector for the Mammalian Expression of Human IgG1 Antibodies with an N297A mutation in the CH2 region known to prevent antibody glycosylation.

The TGEX™ vector series is designed for the rapid expression of antibody molecules by transient gene expression in mammalian cell suspension cultures. TGEX™-HC-hG1[NA]-Zeo expresses the constant region of the human IgG1 heavy chain with an N297A mutation in the CH2 region known to prevent antibody glycosylation (Ju 2014). Expression of full length antibody molecules is achieved by co-transfection with a light chain variable region cloned into one of the TGEX™-LC vector series. Using transient transfection technologies yields of antibody between 20 mg/L and 200 mg/L and more in serum-free conditions can be obtained in just a few days.