MC-Easy Minicircle DNA Production Kit (Includes ZYCY10P3S2T producer E. coli strain)
Non-integrative sustained expression with Minicircle DNA Vector Technology
Product Description
The MC-Easy™ Minicircle DNA Production Kit enables simple, reproducible, and efficient production of high-quality Minicircle DNA. The finely tuned growth and induction media is optimized for minicircle production, and the included ATP-dependent DNase reagent selectively removes genomic DNA without affecting the quality of the minicircle DNA, for clean and effective minicircle production prep after prep.
Workflow
The kit also includes an innovative method for degrading any contaminating genomic DNA using an ATP-dependent DNase reagent that will selectively remove linear genomic and residual parental DNA but will not affect the minicircle DNA. This means clean and effective minicircles every time. The system also comes complete with a special minicircle DNA transfection reagent, MC-Fection, that works well with most cell lines.
Kit Contents
- ZYCY10P3S2T Minicircle DNA Producer Strain, 5 vials of competent cells
- 5X Minicircle Growth medium (200 ml)
- 10X Induction Medium (100 ml)
- Minicircle DNA Re-Purification Kit (5 preps) incl. Minicircle-safe DNAse, ATP, reaction buffer, precipitation buffer, endotoxin free water, and Purification/Concentration columns
- MC-Fection Minicircle DNA Transfection Reagent (100 ul)
The kit is also available without E. coli producer strain ZYCY10P3S2T (cat.# MN910A-1-SBI).
Performance Data
Please note
Parental minicircle plasmids and the ZYCY10P3S2T Producer Bacterial Strain are available for purchase by not-for-profit researchers only. Commercial users may purchase pre-made mcDNA [ready-to-transfect] only. For any other purposes, including the ability to buy the parental MC production system, commercial users should contact us at BioCat for additional details.
Minicircle Service
In cooperation with SBI BioCat also offers custom parental plasmid cloning and mcDNA production to both not-for-profit and commercial end users. Please contact us if you are interested in this service.
Citations and References
Genetically Modified Organism (GVO)
See General Terms.
