MC-Easy Minicircle DNA Production Kit (does not include ZYCY10P3S2T producer E. coli strain)
Non-integrative sustained expression with Minicircle DNA Vector Technology
Product Description
The MC-Easy™ Minicircle DNA Production Kit enables simple, reproducible, and efficient production of high-quality Minicircle DNA. The finely tuned growth and induction media is optimized for minicircle production, and the included ATP-dependent DNase reagent selectively removes genomic DNA without affecting the quality of the minicircle DNA, for clean and effective minicircle production prep after prep.
Workflow
The kit also includes an innovative method for degrading any contaminating genomic DNA using an ATP-dependent DNase reagent that will selectively remove linear genomic and residual parental DNA but will not affect the minicircle DNA. This means clean and effective minicircles every time. The system also comes complete with a special minicircle DNA transfection reagent, MC-Fection, that works well with most cell lines.
Kit Contents:
- 5X Minicircle Growth medium (200 ml)
- 10X Induction Medium (100 ml)
- Minicircle DNA Re-Purification Kit (5 preps) incl. Minicircle-safe DNAse, ATP, reaction buffer, precipitation buffer, endotoxin free water, and Purification/Concentration columns
- MC-Fection Minicircle DNA Transfection Reagent (100 ul)
The kit is also available with E. coli producer strain ZYCY10P3S2T (cat.# MN920A-1-GVO-SBI).
Performance Data
