pMC.EF1-MCS-IRES-GFP-SV40PolyA Parental Minicircle Cloning Vector

Take advantage of Minicircle Technology with pMC.EF1α-MCS-IRES-GFP-SV40polyA, a Parental Minicircle Cloning Vector for episomal expression free of foreign DNA

Product Description

• Episomal expression sustained over weeks
• Foreign DNA-free
• More efficient transfections from small plasmid size
• Unlimited insert size
• Optimized coli minicircle production strain

Get all the advantages of Minicircle Technology with the pMC.EF1α-MCS-IRES-GFP-SV40polyA Parental Minicircle Cloning Vector. This Minicircle Cloning Vector contains the necessary sequences to generate a minicircle, and a moderate EF1α promoter upstream of an MCS, so you can clone-in your gene-of-interest and get medium expression levels in most types, including primary cells and stem cells. In addition, this vector co-expresses GFP from the EF1α promoter for coordinated expression of the GFP reporter with your gene-of-interest, facilitating identification of transfectants via GFP imaging.

Please note

Parental minicircle plasmids and the ZYCY10P3S2T Producer Bacterial Strain are available for purchase by not-for-profit researchers only. Commercial users may purchase pre-made mcDNA [ready-to-transfect] only. For any other purposes, including the ability to buy the parental MC production system, commercial users should contact us at BioCat for additional details.

Minicircle Service

In cooperation with SBI BioCat also offers custom parental plasmid cloning and mcDNA production to both not-for-profit and commercial end users. Please contact us if you are interested in this service.