pPACKH1 HIV Lentivector Packaging Kit

Product Description

Co-transfect HEK293T cells with pHIV-PACK plasmid mix and your pHIV expression product and produce transduction-ready pseudoviral particles.

• Effectively leverage the powerful lentiviral transduction platform with SBI’s optimized HIV-based and FIV-based packaging systems
• Easily generate high-titer lentiviral preparations
• Efficiently deliver most third-generation lentivector expression constructs
• Successfully reach:
  - A wide variety of mammalian cells with VSV-G pseudotyped lentiviral particles
  - A broad range of cell types including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more

Designed to package any of SBI’s RNAi, PathNet, or cDNA Cloning and Expression Lentivectors into pantropic pseudoviral particles, the HIV-based pPACK Packaging System makes it easy to deliver and express lentivector constructs in a wide-range of mammalian cells.

Generate transducible lentiviral particles with your expression construct.

The pPACKH1 Packaging Plasmid Mix consists of three plasmids that produce all the structural and replication proteins needed to transcribe and package an RNA copy of the expression lentivector into recombinant, VSV-G-pseudotyped lentiviral particles. Just co-transfect the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production cells, isolate virus particles, and transduce into target cells. Upon transduction, the lentivector construct becomes stably integrated into the genome of the target cells for long-term expression of genes, miRNAs, shRNAs, etc.

Highly efficient delivery of any lentivector construct.

You can use pseudoviral particles produced by packaging cells to introduce the expression construct into any mammalian cells—including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, etc. Pseudoviral transduction is much more efficient and widely applicable than standard transfection. Once delivered, the lentivector expression construct integrates into the genomic DNA and heritably expresses the siRNA sequence.

Achieve high titers with the 293TN producer cell line.

To facilitate packaging, we offer SBI's 293TN producer cell line which is optimized for effective production of pseudoviral particles at high titers, see link below. The 293TN cells stably express the SV40 large T antigen and neomycin gene products.

Product Components

1. pPACKH1 Packaging Plasmid Mix: Mixture of pPACKH1-GAG, pPACKH1-REV, and pVSV-G plasmids
2. pSIH1-H1-copGFP Positive Control Expression Plasmid

Please note: The HIV-based Packaging Kit is also available in the Lentisuite Deluxe Kit including HEK293TN Cell Line, PEG-It Lentivirus Precipitation Solution and Ultrarapid Lentivirus Titer Kit.

Supporting Data

Lentivirus produced by SBI exhibits broad tropism and robust in vivo transduction efficiency.

Fig.1. SBI’s packaged lentivirus exhibits broad tropism.