NeoSelect pGli1-Luc-Neo Reporter Vector

Product Description

NeoSelect TF Luciferase Reporter Vectors

• Functional assay - It provides functional assays to monitor the activities of TFs in cells
• Quantitative analysis - Activities of TFs are measured through repression of firefly luciferase gene expression
• Stable cell lines – The vectors can be used for establishing stable cells with Neomycin resistance and optional GFP expression

The Luciferase Reporter Vectors have been specially constructed to report the binding activity of a single TF. Multiple copies (at least four) of the cis-acting enhancer element have been inserted into each vector upstream of a minimal TA promoter. Upon vector transfection and cell stimulation, the activated TF is bound to the enhancer element and initiate the transcription of downstream luciferase reporter gene. The chemiluminescent measurement of luciferase activity is proportional to the amount of expressed enzyme and thus the binding activity of the targeted TF. 

Transcription factor (TF) luciferase reporter vectors are available in versions with and without selection markers. Besides the Basic TF Luciferase Reporter version we offer TF luciferase reporter vectors with hygromycin resistance and neomycin resistance, each with optional GFP.

The Transcription factor (TF) luciferase reporter vectors with Neomycin or Neomycin and GFP selection contain a luciferase gene under the control of multiple repeats of TF consensus binding sequence and TATA minimal promoter, allowing researchers to monitor the activity of that promoter in different experimental conditions. Neomycin resistance allows for selection using the antibiotic Neomycin, while GFP provides a visible marker for identifying transfected cells.