DepleteX Human DNA Depletion Kit - Early Access

Boost microbial detection with unbiased human DNA depletion.

Product Description

Human microbiome samples often consist of a mixture of human and microbial DNA. For some samples, contaminating human DNA can be such a large proportion of reads that the sampling of microbial fragments is significantly inhibited. Spending sequencing reads on contaminating human DNA leads to decreased microbial genome coverage, decreased microbial community resolution, and increased sequencing costs.

The DepleteX^TM Human DNA Depletion Kit utilizes a simple CRISPR-based depletion to cut unwanted human molecules that are subsequently removed by an exonuclease treatment. This kit gives you the ability to sequence more microbial reads from your metagenomic samples and get the most comprehensive and unbiased view of the microbiome.

• Unbiased human DNA depletion preserves microbial community
• Improved microbial genome coverage previously obscured by human DNA
• Increased detection sensitivity of low abundance microbial species

Performance Data

Fig.1 Unbiased depletion. Unbiased boost in reads across bacterial species after human DNA depletion. 

Fig.2 Boost microbial reads. 16 fold increase in bacterial reads after human fragment depletion. 

Specifications

This Protocol is compatible with short read sequencing platforms. For long read sequencing compatibility, please contact us.