DepleteX Plus Stranded Total RNA Prep with Microbial RNA Boost (Nasopharyngeal)

Remove abundant human reads to boost the discovery of novel microbes

Product Description

• Simple depletion of human RNA from nasopharyngeal samples
• Improved and agnostic pathogen detection in difficult nasopharyngeal samples with high human content
• Enables detection of viruses at Cq values of up to 30 with 4 million reads
• Includes CRISPRclean Plus Stranded Total RNA Prep Kit (KIT1016-JCG)
  
  

DepleteX Nasopharyngeal Microbial RNA Boost Kit removes highly expressed transcripts in-vitro ahead of sequencing, redistributing sequencing clusters to microbial content and pathogens —allowing you to maximize discovery.

Jumpcode Genomics has developed the DepleteX™ Nasopharyngeal Microbial RNA Boost Kit for researchers interested in investigating the microbial content and presence of pathogens in patient nasopharyngeal samples. 

RNA derived from clinical nasopharyngeal samples is generally poor in quality and quantity. The RNA composition is dominated by those derived from host and commensal microbes, leaving a small fraction of any pathogen of interest. This combination of low input, low-quality RNA, and high host contamination inhibits the sensitivity of mNGS and limits the detection of low-expressing microbes and novel pathogens.

Specifications

Performance Data

Figure 1: Viral Read Counts for Control and Depleted Samples (log-10 scale). Average of 265-fold change in viral read counts between control and depleted samples.