pT7-Guide-IVT Vector

Product Description

T7 driven gRNA Cloning Vector

Features

• BsmB I sites are used for target sequence cloning
• gRNA can be produced via in vitro transcription after the target sequence being cloned
• For mRNA mediated genome editing via microinjection or mRNA transfection, both Cas9 mRNA and gRNA transcript are needed.
  
  

T7 driven Cas9 mRNA can be produced from a separate vector, pT7-Cas9.  A T7 driven Cas9 D10A Nickase mRNA is also available.