pCas-Guide-AAVS1 Vector

Product Description

AAVS1 gRNA vector, validated AAVS1 targeting sequence cloned in pCas-Guide vector.

Features

• Validated CRISPR vector targeting human AAVS1 site
• AAVS1 target sequence: GTTAATGTGGCTCTGGTTCT
• CMV-driven Cas9 and U6-driven AAVS1 gRNA expression

Applications

• Targeted transgene insertion via CRISPR when cotransfected with pAAVS1-puro-DNR (after transgene cloned)
• CRISPR knockin positive control when paired with pAAVS1-RFP-DNR (SKU GE100026)