pCas-Guide-EF1a-GFP Vector

Product Description

pCas-Guide-EF1a-GFP vector (with Cas9 and GFP expression) for genomic target sequence cloning.

Features

All-in-one-vector

• Expresses human codon-optimized Cas9 (C-terminal Myc-DDK tagged)
• BamH I and BsmB I can be used for genomic target sequence cloning
• Expresses gRNA containing the inserted target sequence and the gRNA scaffold
• GFP as a reporter for tracking or sorting transfected cells

   

Performance Data

Fig.1. Fluorescent image of cells transfected with pCas-Guide-EF1a-GFP