Pollen Viability Kit

Product Description

The Pollen Viability Kit (PVK) is used for counting pollen grains and assessing their viability. In contrast to FDA/PI Staining Solution it also contains Pollen Dilution Buffer. Fluorescein Diacetate (FDA) is a non-fluorescent dye that permeates cell membranes, undergoing hydrolysis by the esterase activity of viable cells and producing the green fluorescent compound fluorescein. FDA can be retained in viable cells with intact membranes, acting as a live cell marker. Propidium Iodide (PI), a red membrane-impermeable dye, selectively penetrates dead or damaged cells with compromised membranes. PI is a commonly used dead cell marker binding to DNA and RNA through intercalation. Upon binding, a fluorescence intensity of PI can be increased by 20- to 30- fold higher.

The Pollen Viability Kit contains Fluorescein Diacetate (FDA) 1 x 0.5 ml , Propidium Iodide (PI) 1 x 0.5 ml and Pollen dilution buffer 1 x 20ml.