CD14 Exo-Flow capture kit (Magnetic streptavidin beads, CD14-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain)

Product Description

Go with the flow for easy exosome isolation using FACS

• Enhanced magnetic beads
• Selectively capture billions of exosomes
• Multiple capture antibodies to choose from
• Exo-FITC flow stain
• Modular system to customize captures

Purify Exosomes using FACS: Exo-Flow™ Exosomes are 60 - 180 nm membrane vesicles secreted by most cell types in vivo and in vitro and contain distinct subsets of RNAs and proteins depending upon the cell type from which they are secreted, making them useful for biomarker discovery and functional characterization. Exosomes can originate from any tissue or cell type and end up in a mixed population biofluid. Since exosomes are too small for direct FACS analysis, they must first be captured on a larger surface. The Exo-Flow magnetic antibody bead kits are designed to enable the selective capture for flow sorting to quantify and purify distinct subpopulations of exosomes based on a particular surface marker- "Flow Exometry".

The CD14 Exo-Flow Capture Kit has all the reagents you need to purify exosomes using FACS—magnetic streptavidin beads, CD14-biotin capture antibody, wash and elution buffers, and reversible Exo-FITC stain—you supply the FACS and the optional magnetic stand. The well-validated CD14-biotin antibody and high-quality kit components ensure reliable, reproducible exosome purification based on the presence of CD14 on the exosome surface. And with a larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, assisting with recovery of rare exosome populations.

Note that we also offer reversible Exo-APC as an alternative stain to Exo-FITC.

More than just CD14s

To facilitate the widest range of studies, SBI built the Exo-Flow system to be highly modular. We offer a range of biotinylated antibodies as well as a Basic Exo-Flow Kit with no antibody so that you can use your own biotinylated antibodies.