Rab5b Exo-Flow Capture Kit (Magnetic streptavidin beads, Rab5b-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain)

Product Description

Go with the flow for easy exosome isolation using FACS

• Enhanced magnetic beads
• Selectively capture billions of exosomes
• Multiple capture antibodies to choose from
• Exo-FITC flow stain
• Modular system to customize captures

Purify Exosomes using FACS: Exo-Flow™ Exosomes are 60 - 180 nm membrane vesicles secreted by most cell types in vivo and in vitro and contain distinct subsets of RNAs and proteins depending upon the cell type from which they are secreted, making them useful for biomarker discovery and functional characterization. Exosomes can originate from any tissue or cell type and end up in a mixed population biofluid. Since exosomes are too small for direct FACS analysis, they must first be captured on a larger surface. The Exo-Flow magnetic antibody bead kits are designed to enable the selective capture for flow sorting to quantify and purify distinct subpopulations of exosomes based on a particular surface marker- "Flow Exometry".

The Rab5b Exo-Flow Capture Kit has all the reagents you need to purify exosomes using FACS—magnetic streptavidin beads, Rab5b-biotin capture antibody, wash and elution buffers, and reversible Exo-FITC stain—you supply the FACS and the optional magnetic stand. The well-validated Rab5b-biotin antibody and high-quality kit components ensure reliable, reproducible exosome purification based on the presence of Rab5b on the exosome surface. And with a larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, assisting with recovery of rare exosome populations.

Note that we also offer reversible Exo-APC as an alternative stain to Exo-FITC.

More than just Rab5b

To facilitate the widest range of studies, SBI built the Exo-Flow system to be highly modular. We offer a range of biotinylated antibodies as well as a Basic Exo-Flow Kit with no antibody so that you can use your own biotinylated antibodies. 

Supporting Data

Fig.2. The Rab5b Exo-Flow Capture Kit delivers quantitative, highly selective exosome isolation. Bead flow separation data for exosomes secreted by HEK293 cells and captured using our CDRab5b Exo-Flow Capture Kits. Plots of forward scatter versus FITC intensity show that in the no exosome control, only 0.5% of particles are FITC-positive (left panel), whereas in the exosome-containing sample, 99.3% of particles are FITC-positive (middle panel). The degree of flow separation is shown in the right panel. 
Human serum exosomes were isolated from 250 µL serum using ExoQuick® and the exosome pellet resuspended in 500 µL of 1x PBS. Exosome particles were added as two-fold dilutions starting at 50 µL, and then captured using the biotinylated antibody coupled to Exo-Flow beads. The FITC flow cytometric intensities are then plotted versus the number of exosome particles (determined using NanoSight).

More Supporting Data can be found in the Exo-Flow Product Sheet.