ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

Product Description

• From sample to amplified exoRNAs in a single day
• Isolate exosomes from patient biofluids with the included ExoQuick-TC reagent
• Purify exoRNAs with columns

With cargo that reflects the makeup of their parent cells and their easy isolation, researchers are increasingly turning to exosomes as a source of disease-related biomarkers. The ExoQuick Exosome Isolation and RNA Purification Kit (for Tissue Culture Media) includes everything you need for optimized isolation of exosomal RNAs from serum, plasma, or ascites fluid samples—ExoQuick for fast and efficient exosome isolation and a phenol-free lysis buffer and rapid spin columns to extract RNA from your isolated exosomes. Purified exoRNAs are fully compatible with most downstream applications, such as qPCR, microarray analysis, or NGS.

For exoRNA isolation from other biofluids, try the ExoQuick Exosome Isolation and RNA Purification Kit (for Serum & Plasma).

How it works

Isolate serum exosomes and purify exoRNAs

Supporting Data

Figure 2. Serum exoRNAs prepared using Exosome Isolation and RNA Purification Kit (for Serum & Plasma) deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the ExoQuick Exosome Isolation and RNA Amplification Kit (for Serum & Plasma). The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set. Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.