ExoSure™ Exosome Isolation Kit

Product Description

Exosomes are secreted nanovesicles with diameters ranging from 30 to 150 nm and produced in the endosomal compartment of most eukaryotic cells. They are present in all body fluids under both normal and pathophysiological conditions. They have important roles in mediating intercellular communication by serving as carriers of different biomolecules, including proteins, RNAs, and lipids from one cell to another. These unique properties of exosomes provide opportunities for innovations in diagnosis and treatments. 

Many samples, such as cell culture media, saliva, and urine, contain exosomes at low concentrations. To maximize efficiency, GeneCopoeia provides the ExoSure™ Exosome Isolation System, which combines precipitation and size exclusion chromatography (SEC) techniques, generating high purity and high yields of exosomes. The  ExoSure Exosome Isolation Solution of this kit can be also offered as stand-alone product without collection tubes.

• High yield. Up to 5 x 1010 of purified exosomes per column, depending on cell types and cell culture conditions
• High quality. Preserving extracellular vesicles (EVs) original shape and biological function based on size exclusion chromatography (SEC) method
• Fast and cost-effective. Requires less than 20-minutes of hands-on time, including a convenient column-based clean-up
• Widely used. Available for electron microscopy analysis, Nanoparticle Tracking Analysis (NTA) analysis, nucleic acid and protein analysis, in vitro and in vivo experiments, etc.

Performance Data

Size distribution of ExoSure™-isolated exosomes measured by NTA shows high yield of exosomes isolation

Figure 1. NTA data of ExoSure™-isolated exosomes from HEK293T cells. Size and particle concentration distribution plot of exosomes from HEK293T cells (peak at 101 nm). D90 value is 400nm using ExoSure™ Solution (Cat. No. EP001) (A) and (peak at 90 nm), D90 value is 283nm using ExoSure™ Exosome Isolation Kit (Cat. No. EP002) (B). 

CD63 exosome biomarker western blot assay

Figure 2. CD63 exosome biomarker western blot. Exosome from HEK293T cultured media was isolated by ExoSure™ Exosome Isolation Kit (Cat.No. EP001 and EP002). Both of exosome and HEK293T cell lysates with 10μg(in blue)/5μg(in orange) were detected by anti-CD63 and anti-GAPDH antibody. 

Performance comparison of ExoSure™ with competitor's kit by luciferase assays

Figure 3. Luciferase activity assays. Exosomes were isolated from HEK293T cells transfected with Luciferase-CD63 plasmids using GeneCopoeia ExoSure™ Exosome Isolation Kit and System Biosciences (SBI) ExoQuick-TC® ULTRA EV Isolation Kit. The Luciferase activity of exosomes were tested using Luciferase Assay Kit (GeneCopoeia, Cat. No. LF061).