Quantum Protein Assay Kit - BCA Total Protein Assay Kit

Product Description

This Euroclone protein assay kit is based on the use of bicinchoninic acid (BCA) for the rapid and sensitive detection and quantitation of total protein content. The BCA method is faster and easier than Lowry, with much greater tolerance to interference from non-ionic detergents and buffer salts.

The BCA method combines the biuret reaction, i.e. the reduction of Cu2+ ions to Cu+  by proteins in an alkaline medium with complexation of the latter with bicinchoninic acid. The purple-colored Cu-BCA complex displays a strong absorbance at 562 nm which is proportional to protein concentration over a broad working range.

Protein concentrations are generally determined with reference to standards of a common protein such as Bovine Serum Albumin (BSA). If a more accurate quantitation of an unknown protein is required, the calibration curve has to be constructed using a protein similar to the unknown one.

Features

• Easy to use
• Compatible with most common ionic and non-ionic detergents
• Faster than Lowry method
• Linear working range from 20-2.000 µg/ml (BCA)
• Working solution extremely stable
• Protocol flexibility to increase the sensitivity of the assay
• Cuvette or microplate format

Protocol at a glance

• Prepare working solution (reagent A plus B ratio 5:1)
• Add 2 ml working reagent to 100 µl sample
• Incubate 30 min at 37°C
• Read at 562 nm