EndoFectin™ Expi293 transfection kit (for secreted protein expression)

Product Description

• Specially designed for transfection of the widely-used suspension cell line Expi293F
• Greater transfection efficiency of Expi293F cells than Expifectamine™ 293 (Figure 1).
• Greater protein expression than Expifectamine™ 293 (Figure 2)
• Does not require removal of serum or culture medium, or changing of medium after transfection.

EndoFectin™ Expi293 is a powerful transfection reagent ideally suited for transfection of the suspension cell line Expi293F. Expi293F is the most commonly used suspension HEK293 cell line for transient expression to achieve high cell density. EndoFectin™ Expi293 provides greater transfection efficiency of Expi293 cells than Expifectamine 293 over a range of cell densities. The EndoFectin™ Expi293 kits are designed for high level of secreted (EF017 and EF018) and intracelluar (EF019 and EF020) protein expression and isolation from Expi293F cells. The kits include EndoFectin™ Expi293 transfection reagent, as well as proprietary solutions engineered to enhance protein production. 

Performance Data

Comparison of EndoFectin™ Expi293 and Expifectamine™ 293 in plasmid transfection

Figure 1. Comparison of transfection efficiencies of the suspension cell line Expi293F between EndoFectin™ Expi293 and Expifectamine 293. Expi293F cells were cultured in 125 ml flasks and transfected with a GFP-expressing plasmid. Transfection efficiencies were quantitated as relative fluorescence units (RFU, A) and compared visually using a fluorescence microscope (B).

Figure 2. Comparisons of secreted (A) and intracellular (B) protein expression in suspension cell line Expi293F between the EndoFectin™ Expi293 kit (GeneCopoeia) and the Expifectamine 293 kit (Thermo Fisher). A. Expi293F cells were transfected with a Gaussia luciferase-expressing plasmid. GeneCopoeia transfection solutions or ThermoFisher enhancers were added 16-18 hours after transfection. Gaussia luciferase expression was measured in a luminometer. B. Expi293F cells were transfected with a Firefly luciferase-expressing plasmid. GeneCopoeia transfection solution 3 or Thermo Fisher enhancers were added 16-18 hours after transfection. Firefly luciferase levels were measured in a lluminometer 48 hours after transfection.