VA(Vitamin A) ELISA Kit
Specifications | |
---|---|
Reactivity: | Universal |
Sample Type: | Serum, plasma and other biological fluids |
Sensitivity: | 9.38ng/mL |
Detection Range: | 15.63~1000ng/mL |
Detection Method: | Colorimetric |
Design Principle: | Competitive |
Assay Time: | 2.5h |
Additional Information | |
---|---|
Synonyms: | VA |
Product Description
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VA. During the reaction, VA in the sample or standard competes with a fixed amount of VA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Product Citations
- Aslam Khan, Zia Ul-Haq, Sadia Fatima, Jawad Ahmed, Hussah M. Alobaid, Sheraz Fazid, Nawshad Muhammad, Cecilia Garzon, Yasir Ihtesham, Ijaz Habib, Mahamadou Tanimoune, Khalid Iqbal, Muhammad Arshad, Sher Zaman Safi (2023) Long-Term Impact of Multiple Micronutrient Supplementation on Micronutrient Status, Hemoglobin Level, and Growth in Children 24 to 59 Months of Age: A Non-Randomized Community-Based Trial from Pakistan Nutrients
- Aslam Khan, Zia Ul-Haq, Sheraz Fazid, Sadia Fatima, Nawshad Muhammad, Jawad Ahmed, Salim Manoharadas, Sher Zaman Safi, Ijaz Habib, Cecilia Garzon, Yasir Ihtesham, Fareeda Zahid, Fazal Dad, Tanimoune Mahamadou, Nicola M. Lowe (2023) Effectiveness of locally produced ready to use supplementary food on hemoglobin, anthropometrics, and plasma micronutrients concentrations of 6 to 23 months age children: a non-randomized community-based trial from Pakistan Frontiers in Nutrition
- Catalog Number
E-EL-0135-ELS - Supplier
Elabscience - Size
- Shipping
Blue Ice
Price
501,00 €