VA(Vitamin A) ELISA Kit

Specifications
Reactivity:Universal
Sample Type:Serum, plasma and other biological fluids
Sensitivity:9.38ng/mL
Detection Range:15.63~1000ng/mL
Detection Method:Colorimetric
Design Principle:Competitive
Assay Time:2.5h
Additional Information
Synonyms:VA

Product Description

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VA. During the reaction, VA in the sample or standard competes with a fixed amount of VA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Product Citations

Price
501,00 €
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Please contact:

Dr. Kristin Kördel

Tel. +49 (0) 6221 71415 16

info@biocat.com

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