MitoBright Deep Red Probe Assay Kit

Product Description

MitoBright Deep Red Probe is a lipophilic, deep red fluorescent probe that is cell membrane permeable and can cross the cell membrane of living cells by passive diffusion, resulting in enrichment within mitochondria.

Application: Flow Cytometry
Detection Method:  Fluorometric method, Flow cytometry, Cell Culture
Assay Time: 30 min
Detection Instrument: Fluorescence Microscope, Flow Cytometer
Ex/Em: 644/665
Channel Set: APC
Filter Set: Cy5

Figure 1: HELA cells were co-stained with MitoBright Deep Red Probe and DAPI Reagent (25 μg/mL)

Figure 2: Jurkat cells were induced with 0μM or 5 μM camptothecin for 4h, and then the cells were collected and stained with MitoBright Deep Red Probe. The results showes that the mitochondrial membrane potential decreased with enhanced apoptosis, and the MitoBright Deep Red Probe fluorescence brightness decreased.