MitoBright Green Probe Assay Kit

Product Description

MitoBright Green Probe is a fluorescent dye that can freely pass through cell membranes and is used to label intact mitochondria in living cells.It can specifically bind to negatively charged phospholipids such as cardiolipin on the mitochondrial membrane, and can be used to qualitatively or quantitatively determine the mitochondrial mass and the toxic and damaging effects of free radicals and oxidants on the cellular mitochondria by analyzing the changes in cardiolipin on the mitochondrial membrane. 

Application: Flow Cytometry
Detection Method:  Fluorometric method, Flow cytometry, Cell Culture
Assay Time: 30 min
Detection Instrument: Fluorescence Microscope, Flow Cytometer
Ex/Em: 495/519
Channel Set: FITC
Filter Set: FITC

Figure 1: HELA cells were co-stained with MitoBright Green Probe and DAPI Reagent (25 μg/mL)

Figure 2: Mouse spleen cells were co-stained with MitoBright Green Probe, APC Anti-Mouse CD4 Antibody [GK1.5] (E-AB-F1097E) and Elab Fluor® Red 780 Anti-Mouse CD8a Antibody [53-6.7]