dGH in-Site Service

Examine Edits One Cell at a Time

Product Description

dGH in-Site gives researchers the ability to directly visualize and characterize rearrangements at their edit sites. Using synthetic oligo probes and repeat-free bioinformatic design, dGH in-Site is capable of tracking edit site translocations, inversions, copy number variants, viral integrations and other complex rearrangements with a 2 kb resolution.

Concerns over a safe edit site? dGH in-Site allows researchers to examine their edits one cell at a time without having to worry about PCR biases or misrepresentative sequencing averages from heterogeneous cell populations. As an orthogonal analysis to PCR/sequencing techniques, dGH in-Site assays enable, through direct visualization, definitive measurement of the presence or absence of inserts. This includes genome-wide distribution and orientation of transgenes or inserted segments. When used in combination with our targeted probes and chromosome paints, dGH in-Site assays can provide quantitative, single cell on- vs. off-target outcomes of the delivery and editing process. Moreover, they can be used to track the stability of the edited genome in both clonal isolates and non-clonal populations.

dGH in-Site assay in a CRISPR/Cas edited iPSC, demonstrating both on-target and random integration of insert sequences (yellow) throughout the genome.

Benefits of in-Site

• On- and off-target integration metrics
• Clean integration data, even from complex or heterogeneous cell populations
• A unique, whole genome, orthogonal method of direct visualization of inserts, without bioinformatic prediction of outcomes
• Multi-channel fluorescence for flexible and multiplex assay design
• Available for human, murine, canine, non-human primate and CHO cells

Offering the lowest limit of detection of integrated or genomic DNA targets by fluorescence, dGH in-Site is the most comprehensive tool available for researchers to track genome-wide distribution and orientation of transgenes, inserts and edit site rearrangements.