m6A (N6-methyladenosine) Dot Blot Assay Kit

Product Description

The RayBiotech m6A dot blot assay provides a simple and rapid method of quantifying the global m6A levels in RNA/DNA, urine, serum, plasma, or other nucleic samples collected from humans, rats, and mice.

N6-methyladenosine (m6A) is the most prevalent internal modification of messenger RNAs (mRNAs), affecting their structure, stability, and cellular interactions. In RNA, m6A plays a role in the initiation and progression of different types of human cancers. In DNA, m6A has been identified as an important epigenetic and epitranscriptomic marker in higher eukaryotes. Increasing evidence shows that dynamic m6A modification in genomic DNA is associated with brain functions, glioblastoma, embryogenesis, embryonic stem cell development, and cardiovascular disease. Determining the global changes in m6A levels is necessary to understand both the physiological and pathological states.

How it Works

Graphical representation of m6A quantification by dot blot assay.

Performance Data

Fig.1: Representative image of m6A-specific dot blot assay of RNA, DNA and plasma samples. Total RNA and genomic DNA were isolated from the human lung cancer cell line of A431 and quantified by nanodrop, respectively (A). Plasma isolated from colorectal cancer (CRC) and normal health controls (B). m6A dot blot assay followed this protocol.