Basic Exo-Flow kit without any coupled antibodies (Uncoupled magnetic streptavidin beads, Wash and Elution Buffers, Exo-FITC stain)

Product Description

Go with the flow for easy exosome isolation using FACS

• Enhanced magnetic beads
• Selectively capture billions of exosomes
• Multiple capture antibodies to choose from
• Exo-FITC flow stain
• Modular system to customize captures

Purify Exosomes using FACS: Exo-Flow™ Exosomes are 60 - 180 nm membrane vesicles secreted by most cell types in vivo and in vitro and contain distinct subsets of RNAs and proteins depending upon the cell type from which they are secreted, making them useful for biomarker discovery and functional characterization. Exosomes can originate from any tissue or cell type and end up in a mixed population biofluid. Since exosomes are too small for direct FACS analysis, they must first be captured on a larger surface. The Exo-Flow magnetic antibody bead kits are designed to enable the selective capture for flow sorting to quantify and purify distinct subpopulations of exosomes based on a particular surface marker- "Flow Exometry".

The Basic Exo-Flow Capture Kit allows you to take advantage of our robust Exo-Flow technology with any exosome marker of your choice. Aside from the biotinylated antibody, the kit has all the same reagents as our other Exo-Flow Kits—magnetic streptavidin beads, wash and elution buffers, and reversible Exo-FITC stain—you supply the biotinylated antibody, FACS, and the optional magnetic stand. The high-quality kit components ensure reliable, reproducible exosome purification based on the exosome surface marker of your choice. And with a larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, assisting with recovery of rare exosome populations.

Note that we also offer reversible Exo-APC as an alternative stain to Exo-FITC.

Beyond the Basic Kit

To facilitate the widest range of studies, SBI built the Exo-Flow system to be highly modular. In addition to the Basic Exo-Flow Kit , SBI offers a family of kits with different, well-validated biotinylated antibodies.

Supporting Data

Fig.2. The Exo-Flow Capture Kits deliver quantitative, highly selective exosome isolation. Bead flow separation data for exosomes secreted by HEK293 cells and captured using different Exo-Flow Capture Kits. Plots of forward scatter versus FITC intensity show that in the no exosome control, only 0.5% of particles are FITC-positive (left panels), whereas in the exosome-containing sample, 99.3% of particles are FITC-positive (middle panels). The degree of flow separation is shown in the right panels.

Human serum exosomes were isolated from 250 µL serum using ExoQuick® and the exosome pellet resuspended in 500 µL of 1x PBS. Exosome particles were added as two-fold dilutions starting at 50 µL, and then captured using the biotinylated antibody coupled to Exo-Flow beads. The FITC flow cytometric intensities are then plotted versus the number of exosome particles (determined using NanoSight).