pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector

Product Description

Express multiple transgenes simultaneously

SBI has built and tested a suite of synthetic bidirectional promoter lentivector formats featuring EF1a and PGK in a divergent configuration coordinating the regulation of two or more different transgenes. This promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter and the divergent configuration is stable and resistant to silencing in embryonic stem (ES), trophectoderm stem (TS) and extra embryonic endoderm (XEN) stem cells (MC Golding and MRW Mann, 2011).

The primary benefits of these formats are that researchers can coordinate dual expression of their genes of interest as well as various markers (GFP, RFP, Puro, etc.) without the use of IRES elements which have weak functions when co-expressing two cDNAs and can avoid unidirectional promoter interference issues that sometimes arise in lentivector formats.

Bidirectional lentivector format maps

Sample functional testing data:

Sample virus transduction data after puromycin selection for 4 days in HEK293 cells.