CytoSelect™ 96-Well Phagocytosis Assay (E. coli, Colorimetric Format)

Product Description

The CytoSelect™ 96-Well Phagocytosis Assay, E. coli Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

This Phagocytosis Assay uses enzyme-labeled, E. coli particles as a phagocytosis pathogen; however, it does not involve subjective manual counting of E. coli particles inside cells. Instead external particles are blocked before the colorimetric detection of engulfed E. coli particles (Figure 1). This format provides a quantitative, high-throughput method to accurately measure phagocytosis. The CytoSelect™ 96-well Phagocytosis Assay (E. coli) provides a robust system for screening TLR ligands, phagocytosis activators or inhibitors.

Fig.1. Principle of the CytoSelect™ 96-well Phagocytosis Assay (E.coli Substrate).