CytoSelect™ 96-well Cell Transformation Assay
Product Description
The CytoSelect™ 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.
The kit provides a robust system for screening oncogenes and cell transformation inhibitors.
Please note that cells incubated using this assay may not be recovered intact. For recovery of intact viable cells, consider our Soft Agar Assay with Cell Recovery.
Advantages over Traditional Transformation Assays:
- Time Savings: 7 days vs. 21 days
- 96-well, High-Throughput Format
- No Manual Counting
- Fluorometric Detection
- More Accurate and Precise
- Easy-To-Follow Instructions
Assay Principle
Product Citations
- Andrio E, et al. (2017) Identification of cancer-associated missense mutations in hace1 that impair cell growth control and Rac1 ubiquitylation. Sci Rep.
- Illuzzi, J. L., et al. (2017) Tumor-associated APE1 variant exhibits reduced complementation efficiency but does not promote cancer cell phenotypes. Environ.
- Kuroda T, et al. (2017) Identification of a Gene Encoding Slow Skeletal Muscle Troponin T as a Novel Marker for Immortalization of Retinal Pigment Epithelial Cells. Sci Rep.
- Lee, B. et al. (2017) The long noncoding RNA SPRIGHTLY acts as an intranuclear organizing hub for pre-mRNA molecules. Sci. Adv
- Manzanares MA, et al. (2017) Transforming Growth Factors α and β Are Essential for Modeling Cholangiocarcinoma Desmoplasia and Progression in a Three-Dimensional Organotypic Culture Model. Am J Pathol.
- Catalog Number
CBA-130-CB - Supplier
Cell Biolabs - Size
- Shipping
RT
Price
795,00 €
