CytoSelect™ 24-well Cell Migration Assay (8 µm), Colorimetric

Product Description

• Fully quantify chemotaxis with no manual cell counting
• Measure chemotaxis in less than 6 hours with most cell types
• Membrane inserts are uncoated to allow use with any chemoattractant

Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases.

CytoSelect™ Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

Assay Principle

Fig.1. CytoSelect™ Chemotaxis Assay Principle. Migratory cells move through the polycarbonate membrane toward a chemoattractant underneath the membrane inserts.

Supporting Data

Fig.2. Migration of Human Fibrosarcoma HT-1080 Cells. Cells were seeded at 30,000 cells per well of a 24-well plate and allowed to migrate toward 10% FBS for 4 hours in either the presence or absence of 2µM Cytochalasin D. Migratory cells on the bottom of the polycarbonate membrane were stained (top) and quantified in a fluorescence plate reader (bottom).