Custom iLenti siRNA Construct, Packaged Lentivirus (10e7 IU/ml)

Product Description

Simply provide us with your gene’s accession number for the desired target gene or siRNA target sequence and receive the lentivirus siRNA construct in only 3 weeks.

Once your project is started, our partner Applied Biological Materials (abm) will search their siRNA database of over 20,000 publications covering over 7,000 human and mouse genes. Then four constructs with high knockdown efficiency against the target sequence are designed. If your gene of interest is not yet published, they will design four target sequences using their team’s years of expertise and advanced siRNA software. With four siRNA constructs, it is guaranteed that at least one of the constructs should give over 70% gene knockdown efficiency in appropriate target cells, with over 70% transfection efficiency for any human, mouse, or rat gene.

Don't forget to include our ready-made scramble control construct for your experiments! 

Advantages of iLenti™ siRNA

• iLenti™ vectors are very stable, no special competent cells are required
• iLenti™ uses more potent 27-29 bp oligos
• iLenti™ uses convergent promoters to avoid hairpin loop structures, making sequencing and plasmid growth much easier

iLenti-siRNA Vector

Fig.1. Vector map

Related Links

Infection Protocol
Suggested MOI for Common Cancer Cell Lines

Disclaimer

abm's RNA interference lentiviral vectors contain siRNAs. A dual-convergent promoter system is employed where the sense and antisense strands of the siRNA are expressed by two different promoters rather than in a hairpin loop - this is to avoid any possible recombination events that can occur.

abm guarantees that at least one out of the four siRNA Lentivector constructs purchased in a set will result in over 70% knockdown of gene expression within target cells showing >80% transfection efficiency. If this is not the case, we will provide a one-time replacement of four new constructs with alternative siRNA sequences. To qualify for this replacement, the vectors must be transfected at ≥5 nM and assayed 48 hours post-transfection. Customers must provide adequate data to show >80% transfection efficiency with a positive control, plus additional qPCR data to evaluate the level of gene expression. The replacement set will not covered by the same guarantee, and if these constructs are also considered to be ineffective then, it is most likely the gene is not susceptible to siRNA knockdown.

abm limits its obligation and liability for the success of this technology to providing one replacement of any siRNA lentivector product only. Before sending your inquiry, please make sure you have extensively optimized your experiments, including (where applicable) increasing your MOI, increasing the duration of infection (up to 72 hours), and carrying out clone screening before assaying for knockdown. Please send all replacement inquiries and experimental data to technical@abmgood.com.

Due to the unpredictable nature of customized knockdown, this guarantee does not apply to siRNAs designed 1) against species other than human, mouse, or rat, 2) against regions other than the coding region (CDS), or 3) with customer-provided siRNA target sequences. All sales are final for custom designs.

NOT FOR RESALE without prior written consent of abm. This product is distributed for laboratory research only. Caution: Not for diagnostic use.