SensiFAST Probe dUTP Mix (2x)

Product Description

The SensiFAST™ Probe dUTP Mix has been developed for fast qPCR and is designed for superior sensitivity and specificity with probe detection technology, including TaqMan®, Scorpions® and molecular beacon probes. SensiFAST Probe dUTP Mix has been optimized to prevent crosscontamination, so is ideal for high throughput instruments.

The SensiFAST™ Probe dUTP Mix is a 2x, combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with an antibody mediated hotstart polymerase, dNTP/dUTP mix and MgCl₂ , designed to deliver shorter run times with higher reproducibility, specificity and sensitivity (Fig. 1). SensiFAST™ Probe dUTP Mix is perfect for multiplexing (Fig. 2), allowing more samples to be run in a day with the highest confidence.

Supporting Data

Fig. 1 Sensitivity and reproducibility. A 10 fold serial dilution of human DNA (in quadruplicate) over five orders of magnitude amplified using SensiFAST Probe dUTP Mix. The results illustrate that SensiFAST Probe dUTP Mix gives highly sensitive and reproducible results.

Fig. 2 Multiplexing. A 10 fold serial dilution of human cDNA amplified with four different probes (only one is shown here) in quadruplex using SensiFAST Probe dUTP Mix (red) and a Kit from supplier T (black) using the manufacturers recommended protocol. The results illustrate that SensiFAST Probe dUTP Mix is more sensitive than supplier T in a multiplex reaction.