SensiFAST Probe Direct SuperMix

SensiFAST™ Probe Direct SuperMix is a highly inhibitor-resistant qPCR master mix that provides quick and easy extraction and amplification of DNA from a variety of tissue types

Product Description

• Inhibitor tolerant: optimized proprietary buffer system designed for overcoming common PCR inhibitors in crude
  lysate or unprocessed blood, tissue and plant samples
• Specific: antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
• Sensitive: reliable quantification of low abundance targets and scarce samples
• Reproducible: consistent results between technical replicates for increased confidence in results
• Fast: delivers reproducible, multiplex accurate assay results in as little as 30 minutes

The SensiFAST™ Probe Direct SuperMix is a combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with an antibody- mediated hot-start polymerase, dNTPs and MgCl₂. It has been designed for highly reproducible, accurate assay results in the presence of inhibitors, making it ideal for direct amplification directly from the most challenging samples. SensiFAST™ Probe Direct SuperMix has been tested with a variety of crude samples known to inhibit PCR, from whole blood to proteinase lysed crude DNA extracts, making it ideal for everything from biofluids to animal and environmental samples.

The advanced buffer chemistry and enhancers has been developed for fast qPCR and is designed for superior sensitivity and specificity with probe‑detection technology, including TaqMan^®, Scorpions^® and molecular beacon probes, making SensiFAST Probe Direct SuperMix perfect for multiplexing, allowing more samples to be run in a day with the highest confidence.

Simple, fast amplification
SensiFAST Probe Direct SuperMix has been tested with a variety of crude samples known to inhibit PCR, from whole blood to proteinase lysed crude DNA extracts, making it ideal for everything from biofluids to animal and environmental samples.

Ideal for high throughput
Eliminating costly, time-consuming, cumbersome purification procedures that can lead tosample loss, not only increase the speed and sensitivity of the assay, but also makes SensiFAST Probe Direct SuperMix especially valuable for high-throughput applications with limited starting material, such as gene expression experiments.

Applications

• Gene Expression
• Viral and bacterial testing
• GMO testing
• SNP genotyping
• Mutation detection
• Environmental monitoring

Efficient amplification from different sample types

20% (final volume in reaction) samples of human whole blood containing anticoagulants (EDTA, Heparin and Citrate) and bovine whole blood (EDTA), human cerebrospinal fluid, human urine and cow whole milk were analyses using the SensiFAST Probe Direct SuperMix along with 2% alkaline or proteinase K mouse tail, liver and brain crude DNA extracts. The results illustrate that the reaction efficiency of the SensiFAST Probe Direct SuperMix remained within tolerances (90-110%) in the presence of a wide range of common PCR inhibitors.