MyTaq One-Step RT-PCR Kit

Formulated for highly reproducible first-strand cDNA synthesis and subsequent PCR in a single tube.

• Sensitive – incorporates a blend of high-affinity RT and novel MyTaq HS DNA Polymerase, enabling amplification of low-copy number targets from ≥3 pg total RNA
• Efficient – novel one-step buffer system maximizes the efficiency of both the reverse transcription and PCR steps, delivering improved yield of any target
• Robust – RT tolerates the higher reaction temperatures required to overcome secondary structure, giving reliable detection of even challenging and GC-rich targets
• Specific – MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification
• Flexible – utilizes gene-specific primers for full-length reverse transcription and subsequent PCR amplification of any RNA target
• Convenient – an all-in-one-tube mastermix that improves the speed, convenience and accuracy of RT-PCR

Product Description

MyTaq™ One-Step RT-PCR Kit incorporates the latest advances in buffer chemistry, including Bioline ultra-pure dNTPs, together with a proprietary reverse transcriptase and MyTaq HS, a new generation of antibody-mediated hot-start DNA polymerase. This ensures that MyTaq One-Step RT-PCR Kit enables fast, highly-specific and ultra-sensitive amplification of RNA targets for use in a broad range of downstream applications.

MyTaq One-Step Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative or semi-quantitative analysis of RNA transcription levels. The one-step format uses gene specific primers to maximize amplification of these targets, whilst eliminating non-specific amplification, ensuring highly efficient and sensitive transcription from as little as 3 pg total RNA.

MyTaq One-Step RT-PCR Kit is perfect for the synthesis of double-stranded cDNA products for subsequent gene expression analysis over a broad temperature range, the use of higher temperatures allows reverse transcription through RNA secondary structure, including difficult and GC-rich sequences. Enhanced amplification of these targets ensures high cDNA yields from all RNA, including total RNA, mRNA, in vitro transcribed RNA, snRNA and viral RNA.

Applications

• Gene-expression analysis
• Transcription analysis
• cDNA cloning
• Multiplex RT-PCR

Highly sensitive, full-length RT-PCR amplification of a broad of RNA targets

A 1 kb fragment was amplified in duplicate from serial dilution of mouse total RNA (30 ng, 3 ng, 300 pg, 30pg and 3 pg respectively) using RN18S-1000 primers and the MyTaq One-Step RT-PCR Kit. The completed RT-PCR reactions were analysed on a 1% agarose gel. HyperLadder 50bp (M). The results show that the MyTaq One-Step RT-PCR Kit achieves high–yield, specific amplification from even complex templates.

Introduction to MyTaq